About order on SATA ports

System Performance AHCI/NVMe Performance
1

I have a simple question…
It is necessary to put in order my HHDs and use SATA I port instead of SATA 6 port, for example?
I have all my HDDs / DVD on SATA ports 3 to 6, and 1&2 are free.
Can it significant on lost of performance?

2

The bootable SSD/HDD (with the OS and the boot sector on it) should be connected to the first SATA port, which is offered by the chipset (usually named "port0").

3

In this case, the bootable SSD is NVMe over PCIe.
All the rest are HDD for storage and CD/DVD writer. All on SATA 3-SATA6 ports (because of physical orientation).

4

@snowind :
AFAIK the order of the in-use SATA3 ports for not bootable storage HDDs/SSDs doesn’t matter. It may have an impact on the disk drive letters given by the OS, but this can be manually changed by the user at any time.