@wangyu1221
Provided, that
a) the in-use M.2/PCIe connected SSD is a Samsung one and uses the SATA AHCI protocol,
b) the module named SAMSUNG_M2_DXE.ffs has been properly inserted and
c) the modded BIOS has been successfully flashed,
the M.2 SSD should be bootable in UEFI mode.
If the SSD should not be detected by the BIOS as being bootable, I recommend to create the required EFI boot partition named “Windows Boot Manager” by following “Step 4” of >this< Guide, which has been written for NVMe SSDs, but should work with M.2/PCIe SATA AHCI SSDs as well.