Yes, you need to extract the main BIOS body from the stock capsule via hex or UEFITool, then re-open that BIOS body in UEFITool and replace the stock BIOS region with the BIOS region I modified for you.
It’s easy to do, but not easy to explain if you are not familiar with all these BIOS regions and editing tools etc
Yes, I have CH341A 1.34 software, it’s in this package I always link - http://s000.tinyupload.com/index.php?fil…257455007472602
What are you testing it for, did you have some issue with 1.29 or others? If yes, what is your chip ID, maybe I know the version you need to use or if you need to use another software instead, or need to use different BIOS ID etc.
As for Win10, I only know that everything has issues in Win10, even windows has issues in Win10 
So I doubt that works there, I think I’ve seen reports it does not, so if you need to use CH341A and software I suggest you use Win7
Or, you can use ASProgrammer software, it does work properly in Win10 but it’s not as user friendly - https://github.com/nofeletru/UsbAsp-flash/releases/
Thanks for the ‘how to do’ procedure to get a full Programmer ready BIOS file.
I have tested (under W10) v1.34 and chip detection does fail:
A strange question:
If you are looking at the blue chip logo under the Red Detection failure message you can observe the black chip position on the USB Programmer.
On the black chip schema you can observe the tag which usually does indicate the Pin 1 of the component.
If you compare the tag position (Pin 1 emplacement) it is different of what your pictures does provide into the Guide.
Is the schema logo wrong ?
Please, can you clarify ?
Nota: v1.34 does offer W7 drivers only. 2 variants are offered: SERIAL and PARALLEL.
What variant to use ?
@Lost_N_BIOS
Did you see my Post #34 ?
I have tested ASProgrammer which does seem more recent than any CH341A software version.
The chip is in the Database. The chip connection is OK, but the Chip detection (ID) does fail.
So the both tools (ASProgrammer v4.0 and CH341A Software v1.34) does detect the chip connection (I used PARALLEL USB variant) but does provide "FF" when trying to get the Chip ID !!
Does the only remaining try test to do is to run these tools under W7 instead of W10 ?
On your question about the BIOS chip image on the software, this is incorrect image, the BIOS does NOT go that direction nor in that socket of the programmer. I assume this is just generic “Hey here’s a BIOS chip on a programmer” and is incorrect, even if you use the ending socket.
I did not write any guide, so I am not sure what image you are referring to? The BIOS or SOIC8 pins, should go into the middle socket (not the rear one as the chip is in your images), and pin one is on the lever side (As you see here in the fourth image in part #3 on page one)
For CH341A, all driver is same, you only need to install once for any/all versions. You want PAR driver (CH341PAR.exe)
Yes I saw your post at #34, and I replied to it at post #36. I have tested these chips in ASProgrammer and it’s detected and programs correctly, I’ve verified this on multiple occasions.
Yes, please stop wasting your time in Win10, it’s a fail for everything except leaving backdoors open for anyone wanting to peer in 
@Lost_N_BIOS
I stopped to play with CH341A Programmer under W10 and I got the opportunity to play with a Sony VAIO W7 64bit SP1 system.
Some progress because the BIOS chip is now ‘detected’ but the “MAIN MEMORY” contents does remain “all FF” !!
CH341A Software v1.34 test results:
ASProgrammer v4.0 test results:
Conclusion:
The both tools does detect a BIOS chip, but the both do detect a ‘similar models’ problem:
-> CH341A Software v1.34 does suggest 7 models even if we have selected properly the model MX25L12873F. Why this unuseful suggestion after the proper selection ?
-> ASProgrammer v1.40 does suggest 11 models but the “Find IC” pop up does open with the “IC marking contain:” filled in blank , and does offer no select button.
This does look like a freeze and No Main Memory contents is displayed !
With CH341A Software ALL the Main Memory contents does display FF
So, why this tool does display FF ! The BIOS chip is not a FF filled chip because it does work and boot well !!
Any idea to progress on this very painful “Programmer Tool” validation procedure exercise ?
Thanks for your future comments.
I came across a similar? fault with my old notebook- I couldn’t read the bios chip, a simple old GD25Q32. It took a while until I found out that Samsung had grounded pin 3- IO#2/write protect. Neither a CH341 nor a TL866 plus could handle that: The 866 gave a short circuit warning, the ch341 didn’t say anything but the led went off and the software said “CH341 not connected”.
Disconnected the wire for pin 3 from clamp- everything fine! But I first disconnected the wrong wire (ground,pin 4)- The chip could be ‘read’ just fine but there were only ‘FF’.
@lfb6
Thanks for your interesting experience.
On my side the BIOS chip is very recent as the mboard is a very recent design (ASUS Prime Z390M-Plus).
As you can see on my screenshots posted the CH341A Software does indicate: “Device state: connected”
Device state Connection is quite stable: No alternate red warning ‘not connected’ message with ‘connected’. So, I don’t suspect any ‘bad’ electric contact.
My CH341A Pro Programmer and Clip are also recent.
Do you use a CH341 or a CH341A Programmer ?
Do you use W7 or W10 interface ?
I got a green and a black CH341, both are CH341A, the black one states CH341APro. W10Pro, 1809, 64bit (1709 did work, too- never worried about Win10??). Not reading 128MbChips is possibly software related, never tried newer CH341.exe versions than 1.29, but could today program a Winbond 25Q128FV properly with a CH341A and ASprogrammer 1.3.14.
Yes, recognizing a disconnected programmer is quite easy. But when I disconnected ground (pin4), everything worked fine, "reading" the chip without any complaints but delivering only FFs.
This does confirm the problem is more a hardware problem somewhere and not really a W10 problem.
Do you plan to use CH341A Software v1.34 and ASProgrammer v1.4 for your tests ?
ASprogrammer 1.4 seems to work with both CH341A programmers for Winbond 25Q128FV, too. I think that’s good enough for me…
I never spared a thought on Windows 10 by the way, I always use the programmer/programming software as a normal user (UAC fully enabled) and there was no difference between Windows 7 and Windows 10?
Thanks for the information that for you ASProgrammer v1.4 does work fine under W10 as well as under W7.
On my side I am stuck with no Programmer solution working for the BIOS chip for any of my two ASUS Prime Z390M_Plus mboards.
I didn’t check ASprogrammer 1.4 under W7, I used the CH341A with Software 1.29 and TL866 under Win7.
But: Just to be sure I tried now ASprogrammer 1.4 under W7 in a virtual machine (host W10P x64 1809) and it’s working fine with a DIP8 25Q128FV. I assume that a native W7 would do, too.
Made some experiments, though:
For a DIP8 25Q128FV you can disconnect GND(4), IO3(7), IO2(3)- it doesn’t matter. If you in addition disconnect CLK(6) or IO1(2) or IO0(5) you get everything FF FF (means both chip identification and data).
More interesting, maybe:
If you disconnect CLK(6) or IO1(2) or IO0(5) alone you still get everything FF FF, ASprogrammer14 not complaining at all!
@lfb6
Thanks a lot for sharing your experiments.
You are right I had a contact problem with the Clip Board !!
I do significant progress and finally do succeed today under W10 x64 with ASProgrammer v1.4 as well with CH341A Software v1.34:
@Lost_N_BIOS
I "ll send you a PM about ASUS Z390M-Plus modded BIOS file to use for CH341A programmer.
However, a strange situation because Read/Verify operation does fail with CH341A Software v1.34 and is OK with ASProgrammer v1.40 (test done twice):
So, what Programmer Tool is good ?
Tested with CH341A Software v1.40 the Read/Verify operation is OK under W10:
Nota: I do observe CH341A Software v1.34 Read/Verify operation always does fail at about 50% of the operation. Is it a buffer size limitation bug ?
With my Z390 board I had to use the 1.4 programmer from LostNBios for it to work right. And I used Windows 7 but dunno if it works in Windows 10.
@KedarWolf
With CH341A Software v1.40 Free under W10 interface the Read/Verify operation is OK on my side.
Which Z390 mboard do you tested ?
Z390 Aorus Master. It reads, writes and verifies correctly, and I can boot from the BIOS when done.
I really don’t know, which programmer tool is "best". And: Do you mean hardware or software?
I would never rely on the verify function of the software alone. I program the chip, read it back and compare source- file and the file read from the chip on the PC. (For all Windows versions and back to MS-DOS a simple ‘fc /b source.rom readfromchip.rom’ in a command window will do)
You actually don’t know what happened with these three softwares when you don’t read back the SPI and compare it with the source. When comparing results you should also compare the rom read back from ASp1.4 with the roms read back from both versions of the CH341- programmer. Worst case is that all three are different…
The CH341A is a nice cheap tool, but there is sometimes a lot to worry about (mostly software)!
I don’t remember. It’s a removable socketed BIOS, but I don’t recall what it was detected as.
My BIOS chip is soldered (not socketed removable) and is a MICRONIX MX25L12873F, so may be very different from yours.